Open AccessTHE DEAD-BOX PROTEIN CSHA FROM Staphylococcus aureus Mu 50 EXHIBITS RIBONUCLEASE ACTIVITY
Author(s) -
Huy H. Nguyen,
Ngọc Điệp Nguyễn,
Gia-Buu Tran,
Tan Viet Pham
Publication year - 2021
Publication title -
khoa học và công nghệ
Language(s) - English
Resource type - Journals
ISSN - 2525-2267
DOI - 10.46242/jst-iuh.v44i02.1032
Subject(s) - degradosome , rna , non coding rna , ribonuclease , dead box , rna helicase a , biology , microbiology and biotechnology , chemistry , biochemistry , helicase , gene
DEAD-box proteins play important roles in many RNA processes ranging from RNA synthesis to RNA decay. Furthermore, it has been reported that some bacterial DEAD-box proteins known to be components of the RNA degradosome do not cleave RNA substrates directly. However, the role of DEAD-box proteins in RNA degradation is poorly understood. The present study demonstrated that the DEAD-box protein CshA from the vancomycin-resistant Staphylococcus aureus strain Mu50 possesses RNA degradation activity, ribonuclease activity. Despite having RNA-dependent ATPase activity, CshA did not exhibit RNA helicase activity in vitro. Instead, CshA catalyzed the degradation of single-stranded RNAs of various duplex RNA substrates to form blunt-end RNA products. Thus, we suggest that the ribonuclease activity of the DEAD-box protein CshA may contribute to RNA remodeling in the bacterial RNA degradosome. To our knowledge, this study is the first to report that a DEAD-box protein from a pathogenic bacterium is implicated in multiple ATP-independent activity on RNA, such as degradation.