Open AccessExperience of applying flow cytometry to assess immunologic reactivity in subjects with increased blood vanadium concentration
Author(s) -
Д. Г. Дианова,
Oleg V. Dolgikh,
В. Б. Алексеев
Publication year - 2020
Publication title -
rossijskij immunologičeskij žurnal/russian journal of immunology
Language(s) - English
Resource type - Journals
eISSN - 2782-7291
pISSN - 1028-7221
DOI - 10.46235/1028-7221-312-eoa
Subject(s) - vanadium , flow cytometry , hapten , chemistry , immune system , immunology , physiology , endocrinology , antibody , biology , inorganic chemistry
Environmental pollution with external vanadium results in its accumulation in human biological media and alters ability of human body to properly react to negative impact after exposure to haptens. Aim of the work was to assess immunologic reactivity in subjects bearing increased blood vanadium (hapten) level by using flow cytometry. There were examined adults bearing various blood vanadium concedntration. The test group was comprised by adult subjects (n = 50) having blood vanadium concentration exceeding that one for the upper limit of the reference level and mean reference group (n = 42) corresponding to reference range by 3.7- (р 0.001) and 4.3-fold, respectrively, It was found that subjects with excessively vanadium-contaminated serum were featured with stronger lymphocytes activation, increased count of effector helper T cells, lowered count of regulatory lymphocytes and decreased humoral immune arm, and suppressed p53-dependent control of cellular cycle. Thus, flow cytometry allows to assessing immunologic reactivity in subjects with varying level of blood exogenous chemicals as well as determining body adaptation potential to ewffects exerted by environmental chemical cues.