Open AccessELISA-based comparative analysis of non-specific activity for cytokine-producing protein a gene-positive and negative Staphylococcus aureus strains
Author(s) -
A. I. Fayzullina,
А. В. Зурочка
Publication year - 2020
Publication title -
rossijskij immunologičeskij žurnal/russian journal of immunology
Language(s) - English
Resource type - Journals
eISSN - 2782-7291
pISSN - 1028-7221
DOI - 10.46235/1028-7221-270-eca
Subject(s) - staphylococcus aureus , cytokine , antibody , microbiology and biotechnology , staphylococcus , biology , protein a , antigen , gene , immunology , bacteria , biochemistry , genetics
Here we present the data on examining protein A-gene-positive and -negative Staphylococcus aureus daily broth culture supernaturants for production of cytokine-like substances to determine non-specific activity by using enzyme-linked immunosorbent assay with specific kits (JSC “Vector-Best”, Novosibirsk; LLC “HEMA” (Moscow) to diagnose infections and hormone levels. We found no non-specific cross-activity in all test ELISA kits assessing various Staphylococcus aureus strain supernatants, producing cytokine-like substances identical to human ones, except for HBsAg kit. It may evidence about high specificity for remaining test kits examined and indirectly confirm an assumption that staphylococci are able to secrete proteins specifically reacting mainly with antibodies against human cytokines, and can cross-react with other antigens, but not with antibodies. Cytokine-like or nonspecific activity does not depend on presence or absence of the protein A in Staphylococcus strains examined by us.