Open AccessGelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain
Author(s) -
Jennifer Vandooren,
Nathalie Geurts,
Erik Martens,
Philippe E. Van den Steen,
Steven De Jonghe,
Piet Herdewijn,
Ghislain Opdenakker
Publication year - 2011
Publication title -
world journal of biological chemistry
Language(s) - English
Resource type - Journals
ISSN - 1949-8454
DOI - 10.4331/wjbc.v2.i1.14
Subject(s) - high throughput screening , gelatin , matrix metalloproteinase , chemistry , substrate (aquarium) , enzyme , mutant , biochemistry , microbiology and biotechnology , biology , gene , ecology
To establish a novel, sensitive and high-throughput gelatinolytic assay to define new inhibitors and compare domain deletion mutants of gelatinase B/matrix metalloproteinase (MMP)-9.