Open AccessDeterminaÇão da CL90 e TL90 do isolado IBCB66 de Beauveria bassiana (Ascomycetes: Clavicipitaceae) para o controle de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)
Author(s) -
L. A. G. Barci,
José Eduardo Marcondes de Almeida,
Adriana Hellmeister de Campos Nogueira,
Ângelo Pires do Prado
Publication year - 2009
Publication title -
revista brasileira de parasitologia veterinária/brazilian journal of veterinary parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.484
H-Index - 31
eISSN - 1984-2961
pISSN - 0103-846X
DOI - 10.4322/rbpv.018e1006
Subject(s) - biology , beauveria bassiana , bassiana , acari , veterinary medicine , rhipicephalus microplus , conidium , rhipicephalus , ixodidae , entomopathogenic fungus , larva , beauveria , microbiology and biotechnology , biological pest control , virology , botany , tick , medicine
The objective of this research was to evaluate the pathogenicity and the virulence of the IBCB66 isolate of Beauveria bassiana on infected larvae of Rhipicephalus (Boophilus) microplus. The IBCB66 fungus strain was used as standard isolates of B. bassiana against R. (B.) microplus larvae. The larval bioassay tests using the IBCB66 isolate were carried out to determine the (Lethal Concentration) LC50, LC90, (Lethal Time) LT50 and LT90. The IBCB66 fungus strain was tested at six different concentrations (5x10(6), 10(7), 5x10(7), 10(8), 5x10(8) and 10(9) conidia.mL(-1)) to determine the percentage of larval mortality. In addition, a Probit analysis was also performed. Total larval mortality was observed eighteen days after the beginning of the test in the group treated with 5x10(9) conidia.mL(1). The LC50 and LC90 were 3x10(7) and 5x10(8) respectively and the LT50 and LT90 were 10 and 16 days.