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A simple method to reduce optical cross‐talk effects in chemical imaging with planar optodes
Author(s) -
Mayr Torsten,
Ungerböck Birgit,
Mistlberger Günter,
Borisov Sergey M.,
Klimant Ingo
Publication year - 2012
Publication title -
limnology and oceanography: methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.898
H-Index - 72
ISSN - 1541-5856
DOI - 10.4319/lom.2012.10.101
Subject(s) - planar , optics , optode , luminescence , signal (programming language) , scattering , materials science , computer science , physics , computer graphics (images) , fluorescence , programming language
A simple and universal method to minimize optical cross‐talk in chemical imaging with planar optodes used for metabolic investigation of benthic interfaces is presented. Cross‐talk can lead to smeared images and flattened gradients and therefore to erroneous analytical results. To minimize the cross‐talk effect, an additional optical filter layer is coated on the downside of the transparent support. Cross‐talk light traveling in the support and decoupled by scattering media is absorbed to a higher extent than the virtual signal. The extent of cross‐talk and its reduction was evaluated by imaging the luminescence intensity of sensor layers with sharp edges and isolated points. The influence of cross‐talk on the accuracy of oxygen determination by lifetime measurements was investigated by analyzing aerated and de‐aerated zones of a planar optode placed on a scattering media. The presented method is compared qualitatively with methods previously described to reduce optical cross‐talk effects.