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An automated pH‐controlled culture system for laboratory‐based ocean acidification experiments
Author(s) -
McGraw Christina M.,
Cornwall Christopher E.,
Reid Malcolm R.,
Currie Kim I.,
Hepburn Christopher D.,
Boyd Philip,
Hurd Catriona L.,
Hunter Keith A.
Publication year - 2010
Publication title -
limnology and oceanography: methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.898
H-Index - 72
ISSN - 1541-5856
DOI - 10.4319/lom.2010.8.0686
Subject(s) - seawater , ocean acidification , carbonate , chemistry , ph meter , ph indicator , carbon dioxide , environmental chemistry , chromatography , environmental science , oceanography , geology , organic chemistry
Using a spectrophotometric pH measurement system, an automated 12‐tank culture system was developed, which is capable of maintaining pH levels between 7.51 and 8.00 (local, in‐situ pH) within 0.02 pH units at 15.1°C. The precise control of pH in the individual culture tanks was achieved through the addition of CO 2 ‐enriched seawater to unamended seawater. A feed‐back system, which automatically adjusted the mixing ratio of unamended and CO 2 ‐enriched seawater, ensured that the pH in each tank was kept within 0.02 pH units of target values. After each tank adjustment, the spectrophotometric pH measurement was combined with a dissolved inorganic carbon measurement to automatically re‐calculate carbonate chemistry in each tank. The system was used to study the growth of the geniculate coralline alga Arthrocardia corymbosa at pH levels of 7.52, 7.76, and 7.98.