Premium
Update of “Analysis of 15 N incorporation into D‐alanine: A new method for tracing nitrogen uptake by bacteria” (Veuger et al. 2005, Limnol. Oceanogr. Methods 3:230‐240)
Author(s) -
Veuger Bart,
Middelburg Jack J.,
Boschker Henricus T. S.,
Houtekamer Marco
Publication year - 2007
Publication title -
limnology and oceanography: methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.898
H-Index - 72
ISSN - 1541-5856
DOI - 10.4319/lom.2007.5.192
Subject(s) - ecology , estuary , microbial ecology , marine research , limnology , evolutionary ecology , biology , oceanography , bacteria , geology , genetics , host (biology)
After publication of the original manuscript, the presented method has been applied in various 15N- and 13C-labeling studies to trace incorporation of 15N- and/or 13C into bacterial and total microbial biomass in different coastal sediments (Veuger et al. 2006, Veuger 2006). These applications and analysis of the D-Ala content of some algal- and cyanobacterial cultures yielded valuable information regarding the interpretation of 15N and 13C incorporation into D-Ala and other hydrolysable amino acids (HAAs), which is presented in this update