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Partitioning between benthic and pelagic nitrate reduction in the Lake Lugano south basin
Author(s) -
Wenk Christine B.,
Zopfi Jakob,
Gardner Wayne S.,
McCarthy Mark J.,
Niemann Helge,
Veronesi Mauro,
Lehmann Moritz F.
Publication year - 2014
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.2014.59.4.1421
Subject(s) - water column , denitrification , benthic zone , anammox , nitrate , environmental chemistry , ammonium , sediment , chemistry , pelagic zone , environmental science , hydrology (agriculture) , oceanography , nitrogen , geology , geomorphology , denitrifying bacteria , geotechnical engineering , organic chemistry
We evaluated the seasonal variation of denitrification, anaerobic ammonium oxidation (anammox), and dissimilatory nitrate reduction to ammonium (DNRA) rates in the sediments and the integrative N (and O) isotopic signatures of dissolved inorganic nitrogen (DIN) compounds in the overlying water column of the monomictic Lake Lugano south basin. Denitrification was the dominant NO − 3 reduction pathway, whereas the contribution of anammox and DNRA to total benthic NO − 3 reduction was < 6% and < 12%, respectively. Sedimentary denitrification rates were highest (up to 57.2 ± 16.8 µmol N m −2 h −1 ) during fully oxic bottom water conditions. With the formation of seasonal bottom water anoxia, NO − 3 reduction was partitioned between water column and sedimentary processes. Total benthic NO − 3 reduction rates determined in 15 N‐label experiments and sediment—water interface N 2 fluxes as calculated from water column N 2 : Ar gradients revealed that sedimentary denitrification still accounted for ∼ 40% of total N 2 production during bottom water anoxia. The partitioning between water column and sedimentary denitrification was further evaluated by the natural abundance stable N isotope composition of dissolved NO − 3 in the water column. With anaerobic bottom water conditions, water column NO − 3 concentrations gradually decreased, paralleled by an increase in δ 15 N— and δ 18 O— NO − 3 from approximately 7‰ to 20‰ and from 2‰ to 14‰, respectively. Using a closed‐system (Rayleigh) model, the N and O isotope effects associated with community NO − 3 consumption were 15 ε ≈ 13.7‰ and 18 ε ≈ 11.3‰, respectively. With the assumptions of a relatively low net N isotope effect associated with sedimentary denitrification (i.e., 15 ε sed = 1.5–3‰) vs. a fully expressed biological N isotope fractionation during water column denitrification (i.e., 15 ε water = 20–25‰), our results confirm that 36–51% of reduction occurred within the sediment. The general agreement between the indirect (isotopic) approach and the flux and rate measurements suggests that water column nitrate isotope measurements can be used to distinguish between benthic and pelagic denitrification quantitatively.