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Effect of varying calcium concentrations and light intensities on calcification and photosynthesis in Emiliania huxleyi
Author(s) -
Trimborn Scarlett,
Langer Gerald,
Rost Bjö rn
Publication year - 2007
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.2007.52.5.2285
Subject(s) - emiliania huxleyi , photosynthesis , coccolithophore , calcification , total inorganic carbon , chemistry , botany , carbon fixation , environmental chemistry , biophysics , carbon dioxide , biology , phytoplankton , nutrient , medicine , organic chemistry , pathology
Various protective and metabolic functions for coccolithophore calcification have been proposed such as providing a means to supply CO 2 for photosynthesis. It has also been speculated that calcification helps to dissipate excess energy under high irradiance, thereby circumventing photoinhibition. To address these questions, cells of a calcifying strain of Emiliania huxleyi were grown at three irradiances (30, 300, and 800 µmol photons m −2 s −1 ) in combination with four calcium (Ca) concentrations (0.1, 1, 2.5, and 10 mmol L −1 ) leading to different degrees of calcification in the same strain. Growth rates (µ), particulate organic carbon (POC), and inorganic carbon (PIC) production as well as carbon isotope fractionation (p) were determined. Photosynthetic O 2 evolution and CO 2 and HCO 3 − uptake rates were measured by membrane inlet mass spectrometry (MIMS). The application of this multimethod approach provides new information on the role of calcification in E. huxleyi . Noncalcifying cells showed POC production rates as high as calcifying ones. No differences in p were observed under different Ca concentrations. MIMS measurements indicate that noncalcifying cells can photosynthesize as efficiently as, or even more efficiently than, calcifying ones and that both use HCO 3 − as the main carbon source. The ratio of photosynthetic HCO 3 − uptake relative to net fixation did not differ among cells acclimated to 10 mmol L −1 or to 0.1 mmol L −1 Ca. These results indicate that (1) calcification is not involved in photosynthetic carbon acquisition, and (2) calcification does not provide a means of energy dissipation under high irradiances.