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The influence of ammonium, nitrate, and dissolved oxygen concentrations on uptake, nitrification, and denitrification rates associated with prairie stream substrata
Author(s) -
Kemp Melody J.,
Dodds Walter K.
Publication year - 2002
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.2002.47.5.1380
Subject(s) - nitrification , denitrification , environmental chemistry , ammonium , nitrate , algae , organic matter , nitrogen , benthic zone , chemistry , ecology , biology , organic chemistry
Substrata samples were collected from Kings Creek on Konza Prairie Biological Station (Manhattan, Kansas) and incubated with varying levels of ammonium (NH 4 + ), nitrate (NO 3 − ), and dissolved oxygen (O 2 ) to examine the response of nitrogen (N) uptake and transformation rates. Substrata collected were fine benthic organic matter (FBOM), coarse benthic organic matter, filamentous green algae, bryophytes, suspended particulate organic matter, and epilithic diatoms. Nitrification and denitrification were estimated by use of the nitrapyrin and acetylene inhibition methods, respectively. Ammonium uptake demonstrated Michaelis‐Menten kinetics, with the highest maximum rates (V max ) associated with filamentous green algae (5.90 mg N gdm −1 d −1 ) and epilithic diatoms (4.96 mg N gdm −1 d −1 ). Nitrate uptake did not saturate at the highest NO 3 − addition (25 µg N L −1 ) above ambient when associated with FBOM. Overall, maximum uptake rates of NH 4 + were 10‐fold higher than for NO 3 − . Nitrification response to increasing NH 4 + concentrations was highly variable, depending on the substrata type. Nitrification was lowest under low O 2 conditions, being undetectable when NO 3 − was added but not when NH 4 + was added. Denitrification increased linearly with NO 3 − concentration when associated with epilithic diatoms and FBOM but became saturated at ~20 µg N L −1 above ambient concentrations when associated with filamentous green algae. Samples purged with N 2 gas had the highest rates of denitrification. We predicted stream ecosystem rates using equations derived from the experimental data and substrata mass estimates measured in the field. Substantial temporal variability was predicted in uptake (0–1,300 mg NH 4 + −N m −2 d −1 ; 0–5.2 mg NO 3 − −N m −2 d −1 ), nitrification (0–35 mg NH 4 + −N m −2 d −1 ), and denitrification (0–130 µg N2O‐N m −2 d −1 ) as due to natural variation in water column NH 4 + , NO 3 − , and O 2 concentrations.