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A rapid Chromatographic method for recovery of 15NO2 and NO3‐ produced by nitrification in aqueous samples
Author(s) -
Kator Howard,
Morris Lori Jedlica,
Wetzel Richard L.,
Koepfler Eric T.
Publication year - 1992
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1992.37.4.0900
Subject(s) - nitrification , aqueous solution , contamination , chromatography , environmental chemistry , tracer , chemistry , environmental science , nitrogen , ecology , nuclear physics , biology , physics , organic chemistry
The sensitivity and comparative simplicity of 5 N stable isotopic tracer techniques has been used to quantify rates of nitrification in aquatic systems. However, the most commonly used method for recovery of inorganic oxidized nitrogen compounds from aqueous samples, which is based on liquid‐liquid partitioning, is time consuming and contamination prone. We describe a solid‐phase rapid chromatographic method for recovery of 15 NO 2 − and NO 3 − produced by nitrification in aqueous samples. Compared to liquid‐liquid partitioning, the advantages are significantly reduced processing time and reduced potential for contamination. Typical results are presented for the tidal, freshwater reaches of the James River estuary.