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High‐sensitivity flow cytometer for studying picoplankton
Author(s) -
Frankel Sheila L.,
Binder Brian J.,
Chisholm Sallie W.,
Shapiro Howard M.
Publication year - 1990
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1990.35.5.1164
Subject(s) - picoplankton , autofluorescence , sensitivity (control systems) , laser , flow (mathematics) , fluorescence , optics , environmental science , remote sensing , physics , biology , bacteria , geology , cyanobacteria , genetics , electronic engineering , mechanics , engineering
We developed a relatively small, inexpensive, laser‐based flow cytometer suitable for studying marine picoplankton. The instrument uses an air‐cooled He‐Cd laser with low power consumption and a commercially available, square quartz flow cell. Sensitivity and resolution are sufficient to detect marine prochlorophytes in forward light scatter and autofluorescence and marine bacteria in forward light scatter and stained DNA fluorescence.