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The influence of host contamination on maltose release by symbiotic Chlorella 1
Author(s) -
Douglas A. E.
Publication year - 1988
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1988.33.2.0295
Subject(s) - lernaean hydra , chlorella , percoll , maltose , biology , algae , mixotroph , centrifugation , photosynthesis , chlorella pyrenoidosa , botany , chemistry , biochemistry , microbiology and biotechnology , heterotroph , bacteria , genetics , enzyme
Symbiotic Chlorella cells freshly isolated from green hydra were substantially freed of host cell debris by two methods: washing with 0.05% sodium dodecyl sulfate and centrifugation through a gradient of Percoll. Neither treatment reduced the viability or photosynthetic rate of the algal cells, but maltose release by the native symbionts of green hydra was abolished.