Premium
Determination of amino acid production rate of a marine phytoplankton population with 13C and gas chromatography‐mass spectrometry 1
Author(s) -
Hama Takeo,
Handa obuhiko,
Hama Junko
Publication year - 1987
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1987.32.5.1144
Subject(s) - amino acid , photosynthesis , nitrogen , phytoplankton , chemistry , isotopes of carbon , population , carbon fibers , gas chromatography , carbon dioxide , environmental chemistry , food science , botany , biochemistry , biology , chromatography , total organic carbon , organic chemistry , nutrient , materials science , composite number , composite material , demography , sociology
Combined 13 C and gas chromatography ‐mass spectrometry (GC‐MS) were applied to determine the rate of amino acid production of marine phytoplankton populations. The accuracy of the estimate of the 13 C ratio in each amino acid using the relative intensity of isotope peaks was investigated with 13 C‐enriched amino acid standards. Production rates of 12 amino acids of a natural phytoplankton population were determined. The gross amino acid composition of photosynthetic products is generally comparable with previous reports. Production of amino acid at night was confirmed by the increase in 13 C atom%; glucose appeared to supply carbon for amino acid production at night. The ratio of carbon : amino acid nitrogen in photosynthetic products showed a marked diurnal change, due mainly to the loss of glucose carbon at night and the continuing uptake of nitrogen.