Premium
The specific absorption coefficients of chlorophyllide a and pheophorbide a in 90% acetone, and comments on the fluorometric determination of chlorophyll and pheopigments 1
Author(s) -
Lorenzen C. J.,
Newton Downs J.
Publication year - 1986
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1986.31.2.0449
Subject(s) - pheophorbide a , pheophytin , acetone , chemistry , absorption (acoustics) , chlorophyll , chlorophyll a , chromatography , algae , botany , biochemistry , organic chemistry , photosynthesis , biology , materials science , photosystem ii , composite material
Chromatographically pure chlorophyllide a and pheophorbide a were obtained from cultured algae by thin layer chromatography. After 7–10 cell divisions in the presence of 14 C, the cells (and all inclusions) used for the study were uniformally labeled. Measurement of the optical densities and radioactivities of chlorophyll a and these other pigments permits calculation of the specific absorption coefficients for the latter. The specific absorption coefficients measured were: pheophytin a −49.5; chlorophyllide a −128; and pheophorbide a −69.8.