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Micromethod for lipids in aquatic invertebrates 1
Author(s) -
Gardner Wayne S.,
Frez William A.,
Cichocki Elizabeth A.,
Parrish Christopher C.
Publication year - 1985
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1985.30.5.1099
Subject(s) - chromatography , gravimetric analysis , extraction (chemistry) , chemistry , thin layer chromatography , gas chromatography , fish <actinopterygii> , dry weight , flame ionization detector , solvent , environmental chemistry , biology , botany , fishery , biochemistry , organic chemistry
Microtechniques with disposable, calibrated, capillary pipets were developed to measure amounts and classes of lipids in individual Lake Michigan benthic invertebrates. After lipids from an animal were partitioned into 100 µ l of extraction solvent and purified, measured portions were weighed with an electrobalance. Lipids in some samples were also characterized by thin layer chromatography with flame ionization detection (TLC‐FID). The extraction method was calibrated gravimetrically with cod liver oil and evaluated with a dry, ground fish sample. Lipid content was proportional ( r = 0.99) to fish tissue dry weight over the range of 0.3–13 mg. TLC‐FID measurements correlated well ( r = 0.98) with gravimetric analysis for portions of extracts of individual amphipods ( Pontoporeia hoyi ) and oligochaetes ( Stylodrilus heringianus ). TLC‐FID analysis indicated that <5% of the measured gravimetric weights was due to the inclusion of nonlipid material in the extracts.