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Effects of flow rate on fluorescence in vivo during continuous measurements on Gulf of Mexico surface water 1
Author(s) -
Sweet Stephen T.,
Guinasso Norman L.
Publication year - 1984
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1984.29.2.0397
Subject(s) - fluorometer , fluorescence , volumetric flow rate , intensity (physics) , residence time (fluid dynamics) , flow (mathematics) , analytical chemistry (journal) , chemistry , optics , chromatography , geology , physics , mechanics , geotechnical engineering
The intensity of in vivo fluorescence changed with the rate of sample flow during continuous measurements made in the Gulf of Mexico. Most previous measurements have indicated no relationship between fluorescence intensity and sample flow rate. The effect was not observed at flow rates between 0 and 250 cm 3 ·min ‒1 ; however, above 500 cm 3 ·min ‒1 fluorescence increased promptly when flow rate increased. Residence time of sample in the illuminated zone of the fluorometer ranged from 0.29 s (at 1,500 cm 3 ·min ‒1 ) to 1.7 s (at 250 cm 3 ·min ‒1 ). This flow rate effect implies that phytoplankton fluoresce with an intensity which varies as a function of illumination time. The Kautsky induction effect may provide an explanation for the observed variation.