Premium
Chlorophyll determinations with filter fluorometer: Lamp/filter combination can minimize error 1
Author(s) -
Baker Karen S.,
Smith Raymond C.,
Nelson James R.
Publication year - 1983
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1983.28.5.1037
Subject(s) - fluorometer , calibration , filter (signal processing) , chlorophyll , chlorophyll a , remote sensing , chemistry , analytical chemistry (journal) , environmental science , optics , mathematics , chromatography , statistics , computer science , physics , fluorescence , geology , biochemistry , organic chemistry , computer vision
A correct lamp/filter combination is essential in order to optimize the accuracy of chlorophyll measurements made with filter fluorometers. For example, a nonoptimum lamp in a Sequoia‐Turner 111A fluorometer can affect the acid ratio. The magnitude of the acid ratio factor dictates the size of the error propagation from calibration to chlorophyll determination. A low value of the acid ratio causes a greater uncertainty in the chlorophyll determination. As long as the acid ratio is ≥2.0, any error in the acid ratio, which is determined at the time of instrument calibration, is not amplified in the calculations of chlorophyll concentration.