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A new incubator for measuring photosynthetic activity of phototrophic microorganisms, using the amperometric oxygen method 1
Author(s) -
Zevenboom Wanda,
Post Anton F.,
Van Hes Udo M.,
Mur Luuc R.
Publication year - 1983
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1983.28.4.0787
Subject(s) - incubator , photosynthesis , phototroph , irradiance , algae , oxygen , oxygen evolution , amperometry , environmental science , chemistry , botany , environmental chemistry , biology , physics , optics , electrochemistry , microbiology and biotechnology , organic chemistry , electrode
A new incubator was developed for measuring photosynthesis under strictly controlled conditions, using the amperometric oxygen technique. The most important feature is an incubation vessel equipped with a cooling circuit to keep temperature constant at any desired value ±0.05°C. The shape of the vessel and the virtually parallel light bundle allow determination of the light irradiance experienced by the algae. Samples can be exposed to five different irradiance values (0–1,250 W · m −2 ) simultaneously, without affecting light quality. The photosynthetic response is continuously registered for the study of time‐dependent processes in oxygen evolution.