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A method for recovering nanoplankton from filters for identification with the microscope: The filter‐transfer‐freeze (FTF) technique 1
Author(s) -
Hewes Christopher D.,
Holm-Hansen Osmund
Publication year - 1983
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1983.28.2.0389
Subject(s) - microscope , plankton , phase contrast microscopy , optical microscope , filter (signal processing) , interference (communication) , microscopy , phase (matter) , materials science , optics , differential interference contrast microscopy , filter paper , biological system , chromatography , computer science , chemistry , biology , physics , scanning electron microscope , telecommunications , ecology , computer vision , channel (broadcasting) , organic chemistry
A method has been developed to concentrate nanoplankton in natural water samples for transfer to solid surfaces for light microscopy. Marine phytoplankton are used to demonstrate the convenience and ease of our filter‐transfer‐freeze (FTF) technique in recovering filtered material, which can be mounted and ready for examination within 30 min. Samples can be observed with standard or inverted microscopes using phase contrast, Nomarski interference, or other optical systems. The micro‐ and nanoplankton mounted on slides prepared by the FTF technique retain their cellular integrity and lifelike appearance.