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Sample preparation for quantitative autoradiography of phytoplankton 1
Author(s) -
Smith Ralph E. H.,
Kalff J.
Publication year - 1983
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1983.28.2.0383
Subject(s) - tracer , polycarbonate , sample preparation , leakage (economics) , calibration , chromatography , chemistry , environmental science , materials science , mathematics , physics , composite material , statistics , nuclear physics , economics , macroeconomics
A method of sample preparation for quantitative autoradiography of 33 PO 4 uptake by phytoplankton avoids problems of cell damage and tracer leakage common to earlier techniques. A combination of very brief fixation by Lugol’s iodine with collection of cells on polycarbonate filters minimized leakage of tracer while cells remained intact in the preparation of track (thick‐layer) autoradiograms. Counting efficiency differed from that in other applications of the track technique, indicating the importance of calibration in quantitative autoradiography. Quantitative yield was consistent among species with the modified technique.