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Preparation of 15 N‐labeled phytoplankton samples for optical emission spectrometry 1
Author(s) -
Kristiansen S.,
Paasche E.
Publication year - 1982
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1982.27.2.0373
Subject(s) - kjeldahl method , phytoplankton , analytical chemistry (journal) , chemistry , plankton , spectrometer , environmental chemistry , optics , physics , nitrogen , biology , ecology , organic chemistry , nutrient
A method is described whereby the 15 N content of phytoplankton on a filter can be determined in an optical emission spectrometer after Kjeldahl digestion, microdiffusion, and Dumas oxidation. Total N content can be measured in the same sample, and the procedure offers some additional advantages. Values of 15 N content obtained in this way were the same, within the limits of analytical error, as those obtained by direct Dumas oxidation of 15 N‐labeled plankton samples.