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Zooplankton filtering rates: Error due to loss of radioisotopic label in chemically preserved samples
Author(s) -
Holtby L. Blair,
Knoechel Roy
Publication year - 1981
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1981.26.4.0774
Subject(s) - zooplankton , algae , iodine , chemistry , cladocera , yeast , preservative , ethanol , chromatography , weight loss , zoology , food science , biology , environmental chemistry , botany , biochemistry , ecology , organic chemistry , obesity , endocrinology
Zooplankton fed 32 P‐labeled yeast or 14 C‐labeled algae were preserved with Formalin, ethanol, or Lugol’s iodine and the subsequent loss of labeled materials was followed by analysis of sample filtrates. The commonly used combination of 32 P‐labeled yeast and Formalin preservation produced maximal loss in both magnitude and duration, reaching a value of 73% loss after 3 days; ethanol preservation resulted in only 5% loss for the same food. Lugol’s iodine yielded the best results for animals fed 14 C‐labeled algae, resulting in a 40% loss that stabilized within 3 h. Nonchemical preservation (heat‐killing and drying) produced filtering rates comparable with those of the best chemical preservative.