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Microfluorometric method to measure ammonium in natural waters 1
Author(s) -
Gardner Wayne S.
Publication year - 1978
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1978.23.5.1069
Subject(s) - seawater , ammonium , o phthalaldehyde , chemistry , chromatography , fluorescence , measure (data warehouse) , analytical chemistry (journal) , high performance liquid chromatography , derivatization , biology , ecology , organic chemistry , physics , computer science , quantum mechanics , database
Ammonium can be measured fluorometrically after a simple chromatographic separation from amino acids and reaction with o‐phthalaldehyde. The method (<0.5‐ml sample required) is sensitive down to 0.2 µ m NH 4 + in seawater. Fluorescence is proportional to concentration over the range of 0–100 µ m NH 4 + and analysis time is 6 min per sample.
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