Carotenoid interference in the spectrophotometry determination of chlorophyll degradation products from natural populations of phytoplankton 1 | Zendy

Riemann B. | Zendy

Premium

Carotenoid interference in the spectrophotometry determination of chlorophyll degradation products from natural populations of phytoplankton 1

Author(s) -

Riemann B.

Publication year - 1978

Publication title -

limnology and oceanography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.7

H-Index - 197

eISSN - 1939-5590

pISSN - 0024-3590

DOI - 10.4319/lo.1978.23.5.1059

Subject(s) - pheophytin , chlorophyll , chlorophyll a , fucoxanthin , absorption (acoustics) , spectrophotometry , chemistry , carotenoid , acetone , chlorophyll c , photochemistry , pigment , botany , environmental chemistry , chromatography , chlorophyll fluorescence , photosynthesis , biology , food science , materials science , biochemistry , organic chemistry , photosystem ii , composite material

Pigments extracted from natural populations of diatoms showed increased absorption between 600–750 nm after acidification with HCl at concentrations above 3 × 10 M, both in 90% acetone and absolute methanol. This increase in absorption, due to spectral changes in fucoxanthin, made reliable spectrophotometric distinction between chlorophyll a and pheopigments impossible. Changes in absorption spectra of chlorophyll a , pheophytin a, diatoxanthin, and carotene were of minor importance. Corrections for chlorophyll degradation products should be made with care to ensure that the presence of pheopigment a is legitimate and not a function of the ratios of epoxidic carotenoids to chlorophyll a .

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research