Premium
Carotenoid interference in the spectrophotometry determination of chlorophyll degradation products from natural populations of phytoplankton 1
Author(s) -
Riemann B.
Publication year - 1978
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1978.23.5.1059
Subject(s) - pheophytin , chlorophyll , chlorophyll a , fucoxanthin , absorption (acoustics) , spectrophotometry , chemistry , carotenoid , acetone , chlorophyll c , photochemistry , pigment , botany , environmental chemistry , chromatography , chlorophyll fluorescence , photosynthesis , biology , food science , materials science , biochemistry , organic chemistry , photosystem ii , composite material
Pigments extracted from natural populations of diatoms showed increased absorption between 600–750 nm after acidification with HCl at concentrations above 3 × 10 M, both in 90% acetone and absolute methanol. This increase in absorption, due to spectral changes in fucoxanthin, made reliable spectrophotometric distinction between chlorophyll a and pheopigments impossible. Changes in absorption spectra of chlorophyll a , pheophytin a, diatoxanthin, and carotene were of minor importance. Corrections for chlorophyll degradation products should be made with care to ensure that the presence of pheopigment a is legitimate and not a function of the ratios of epoxidic carotenoids to chlorophyll a .