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Fulvic acid interferences on ATP determinations in sediments 1
Author(s) -
Cunningham H. Wilson,
Wetzel Robert G.
Publication year - 1978
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1978.23.1.0166
Subject(s) - oxalic acid , cationic polymerization , chemistry , precipitation , fulvic acid , organic acid , inorganic chemistry , biochemistry , organic chemistry , humic acid , fertilizer , physics , meteorology
Organic and cationic inhibitions of ATP determination in sedimentary systems are large. Cationic inhibitions are reversible and can be successfully removed by cation exchange resins or by precipitation with oxalic acid. Acid‐soluble organic compounds (fulvic acids) complex with extracted ATP; 70–80% of the ATP will not react with the luciferin‐luciferase complex. Inhibitions by the fulvic acid are not reversible. When combined with other metabolic parameters, ATP concentrations provide a good estimate of the metabolic activity of a community.