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Improved filtration procedures for freshwater [ 35 S]SO 4 uptake studies 1
Author(s) -
Jordan Marilyn J.,
Daley Ralph J.,
Lee Kenneth
Publication year - 1978
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1978.23.1.0154
Subject(s) - filtration (mathematics) , radiochemistry , chemistry , environmental science , environmental chemistry , mathematics , statistics
Procedural improvements suggested in the use of [ 35 S]SO 4 uptake as a possible measure of microbial production include: prompt filtration and sterilization of stock solutions, use of polycarbonate Nuclepore filters, prewetting filters, addition of Na 2 SO 4 to samples before filtration, rinsing filters with 1 g liter −1 (0.01 M) Na 2 SO 4 , removal of filter edges before liquid scintillation counting, and dissolving Nuclepore filters in phenethylamine before adding scintillation cocktail. This procedure results in control filter counts of 0–200 cpm above background at isotope concentrations of up to 2.0 µ Ci ml −1 of sample.