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The effect of acidification on measurements of microbial uptake of radioactive glucose in freshwater
Author(s) -
Ramsay Angela J.
Publication year - 1976
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1976.21.6.0922
Subject(s) - assimilation (phonology) , replicate , heterotroph , environmental chemistry , bacteria , chemistry , biology , philosophy , linguistics , statistics , mathematics , genetics
Untake and retention of 14 C‐glucose by bacteria in freshwater was studied using the heterotrophic potential method. Fixing the organisms with acid and immediately cooling to 0°C eliminated the loss of labeled material frequently observed when organisms were acidified only. The cooling treatment also reduced the variability between replicate kinetic parameters calculated from assimilation data.