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A method for the spectrophotometric measurement of chlorophyll a and pheophytin a in benthic microalgae 1
Author(s) -
Tett Paul,
Kelly Mahlon G.,
Hornberger George M.
Publication year - 1975
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1975.20.5.0887
Subject(s) - pheophytin , periphyton , acetone , chlorophyll a , benthic zone , chlorophyll , chemistry , methanol , botany , algae , geology , oceanography , photosystem ii , biology , organic chemistry , biochemistry , photosynthesis
A method is described that allows the measurement of river periphyton chlorophyll a and pheophytin a both on rocks and in soft sediments. The method involves successive extractions directly from frozen samples with boiling methanol. Pigments are estimated by optical density measurements before and after acidification. Methanol is superior to acetone for the distinction of chlorophyll a and pheophytin a by this means.