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Some observations on the fluorometric determination of glucose in freshwater
Author(s) -
Jones J. G.,
Simon B. M.
Publication year - 1975
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1975.20.5.0882
Subject(s) - dilution , chemistry , reproducibility , chromatography , substrate (aquarium) , isotope dilution , environmental chemistry , kinetics , profundal zone , geology , thermodynamics , oceanography , mass spectrometry , physics , quantum mechanics , littoral zone
The sensitivity of the fluorometric determination of glucose is occasionally severely reduced by quenching in profundal samples. The reproducibility of the method in such samples was investigated, as were the effects of dilution of mud samples and substrate concentration on the apparent glucose uptake rate. Observations on the disappearance of glucose from solution in the presence of mud showed that dilution of the mud could produce serious overestimates of the uptake rate. Analysis of the kinetics of uptake in undisturbed mud cores suggests that rough estimates of the kinetic parameters may be obtained for samples where the addition of radioactive tracers is undesirable.