Premium
Direct counts of aquatic bacteria by a modified epifluorescence technique 1
Author(s) -
Daley Ralph J.,
Hobbie John E.
Publication year - 1975
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1975.20.5.0875
Subject(s) - acridine orange , fluorescence microscope , fluorescence , distilled water , bacteria , biology , chromatography , chemistry , optics , biochemistry , physics , genetics , apoptosis
Various excitation and emission filters, microscope lamps, membrane filters, and field storage techniques used for direct counting of bacteria by epifluorescence microscopy have been compared. A rapid, simple modification of the Acridine Orange procedure using a new light filter combination, Sartorius cellulose membrane filters, and distilled water rinse gave the brightest cell fluorescence, darkest background, least fading, and highest counts for both estuarine and freshwater samples. Field fixation with Formalin permits storage of bacterial samples for 1 to 2 weeks without changes in bacterial numbers.