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Some observations on direct counts of freshwater bacteria obtained with a fluorescence microscope
Author(s) -
Jones J. Gwynfryn
Publication year - 1974
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1974.19.3.0540
Subject(s) - acridine orange , acridine , fluorescence , bacteria , fluorescein , fluorescein isothiocyanate , chromatography , chemistry , staining , biology , optics , organic chemistry , physics , genetics
The use of different fluorochromes for direct counts of bacteria in water was compared. The two dyes in most common use, acridine orange (AO) and fluorescein isothiocyanate (FITC) were compared with an acridine derivative, euchrysine 2GNX (E‐2GNX). Minor changes in technique could produce significant differences in counts. The acridine based dyes were easier to apply than FITC, and of these, E‐2GNX gave consistently higher counts, all at a final dye concentration of 5 mg liter −1 with a contact time of 3 min. Details of the methodology should be consulted since changes can produce opposite results.