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An evaluation of liquid scintillation counting techniques for use in aquatic primary production studies 1
Author(s) -
PUGH P. R.
Publication year - 1973
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1973.18.2.0310
Subject(s) - liquid scintillation counting , chromatography , toluene , chemistry , suspension (topology) , reagent , mathematics , organic chemistry , homotopy , pure mathematics
Methods evaluated for the liquid scintillation counting of samples from aquatic primary production studies include the counting of intact filters and cells in a toluene fluor, solubilization and counting of membrane filters and cells in various reagents, and counting of a suspension of cells mixed with the fluor. A 1:1 Triton X‐100:toluene fluor was very effective for the suspension of cells and gave high counting efficiencies. Up to 5 ml of sample could be mixed with the fluor, but this was considered inadequate for most primary production studies. The filter standardization method for the counting of intact filters was investigated further and found to be applicable over a wide range of conditions but only accurate when the weight of algae on the filters was small (< 1 mg). Direct solubilization of the filters and cells in a naphthalene‐dioxane or 2‐methoxyethanoltoluene fluor were the simplest, most accurate, and economical methods for primary production studies; the latter dissolved both wet and dry cellulose nitrate membrane filters and gave excellent replicate counting.