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SEPARATING CONSTITUENTS OF NATURAL PHYTOPLANKTON POPULATIONS BY CONTINUOUS PARTICLE ELECTROPHORESIS 1
Author(s) -
BAYNE DAVID R.,
LAWRENCE JOHN M.
Publication year - 1972
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1972.17.3.0481
Subject(s) - phytoplankton , particle (ecology) , field flow fractionation , seston , algae , fractionation , buoyancy , algal bloom , environmental chemistry , chemistry , chromatography , biology , ecology , nutrient , physics , quantum mechanics
The Beckman continuous particle electrophoresis (CPE) system was used to isolate components of mixed phytoplankton suspensions. Twenty‐one freshwater phytoplankton genera were tested. All algal cells examined migrated toward the anode and different mobilities frequently resulted in fractionation of mixtures of different genera. Organic debris, clay particles, and bacteria often exhibited distinct mobilities that led to their separation from other constituents. Tris (hydroxymethyl) amino methane, p H 9.2, provided greater particle mobility and better resolution than sodium diethylbarbiturate, p H 8.6. Increase in buffer p H tended to make algal particles more negative and increased mobility. A direct relationship existed between applied field gradient and particle mobility. Resolution generally improved at higher field gradients. The effectiveness of the separation was limited by such algal properties as motility, size, formation of aggregates, and buoyancy.