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PHOSPHORUS‐33 AUTORADIOGRAPHY USED TO MEASURE PHOSPHATE UPTAKE BY INDIVIDUAL ALGAE
Author(s) -
FUHS G. WOLFGANG,
CANELLI EDMONDO
Publication year - 1970
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1970.15.6.0962
Subject(s) - phosphorus , algae , diatom , phosphate , phytoplankton , population , environmental chemistry , chemistry , biology , nutrient , botany , ecology , biochemistry , organic chemistry , demography , sociology
Diatoms from pure cultures and samples of lake phytoplankton were labeled with and autoradiographs were prepared after concentration by centrifuge and membrane filter techniques. Conditions for labeling at a suitable intensity (1–3 dpm/1,000‐ µ 3 cell volume) were determined. The method is suitable for the detection of nonspecific binding of phosphorus by organic materials and of differential phosphorus uptake by members of a mixed population. It also was used to determine continuity of phosphorus uptake in a cultured diatom.