Premium
A LIQUID SCINTILLATION PROCEDURE FOR DETERMINING THE EFFECT OF SIZE ON SELF‐ABSORPTION OF 14 C IN DAPHNIA PULEX 1
Author(s) -
WARD F. J.,
WONG BRIAN,
ROBINSON G. G. C.
Publication year - 1970
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1970.15.4.0648
Subject(s) - daphnia pulex , absorption (acoustics) , liquid scintillation counting , chromatography , cladocera , digestion (alchemy) , carbon 14 , daphnia , solubilization , pulex , chemistry , biology , biochemistry , ecology , crustacean , optics , physics , quantum mechanics
Self‐absorption coefficients for 14 C in Daphnia pulex ranging from 1.1 to 2.9 mm long were determined with a liquid scintillation counting procedure. Coefficients were calculated from two regressions: that of the radioactivity of labeled whole animals on size and that of the radioactivity of labeled animals digested for 24 hr at 60C in the solubilizer NCS on size. Self‐absorption coefficients increased from 1.23 for animals with a mean body carbon content of 6.6 µ g C/ individual to 2.73 for animals with 44.7 µ g C/individual. If quenching is moderate and digestion complete, specific activities of small animals can be determined directly using an efficient solubilizer, eliminating the need to use self‐absorption coefficients.