Premium
EFFECT OF HYDROSTATIC PRESSURE ON SYNTHESIS OF PROTEIN, RIBONUCLEIC ACID, AND DEOXYRIBONUCLEIC ACID BY THE PSYCHROPHILIC MARINE BACTERIUM, VIBRIO MARINUS 1
Author(s) -
Albright Lawrence J.,
Morita Richard Y.
Publication year - 1968
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1968.13.4.0637
Subject(s) - psychrophile , dna synthesis , hydrostatic pressure , rna , protein biosynthesis , biology , dna , bacteria , biochemistry , microbiology and biotechnology , gene , genetics , physics , thermodynamics
The temperature and hydrostatic pressure maxima for growth of the marine psychrophilic bacterium, Vibrio marinus MP‐4, were 20C and 425 atm. The effects of pressures of 200, 400, 500, and 1,000 atm at 15C on synthesis of net protein, RNA, and DNA by growing cells of V. marinus MP‐4 were determined. A pressure of 1,000 atm completely inhibited protein and DNA synthesis but allowed RNA synthesis decreased for approximately 60 min and then resumed the 1‐atm rates, whereas DNA synthesis was unaffected. Pressures of 400 and 500 atm immediately lowered the rate of protein synthesis, whereas RNA synthesis was unaffected by 400 atm. At 500 atm, RNA synthesis continued at the 1‐atm rate for 30 min and then shifted to a lower rate. Synthesis of DNA, at 400 and 500 atm, continued at the 1‐atm rate for approximately 60 min after pressurization, then gradually shifted to lower rates. The data suggest that the primary effect of pressures from 400 to 500 atm was to lower the rate of protein synthesis, which in turn may have lowered the rates of RNA and DNA synthesis.