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A MICROBIOLOGICAL ASSAY FOR VITAMIN B 12 IN SEAWATER USING RADIOCARBON 1
Author(s) -
Gold Kenneth
Publication year - 1964
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1964.9.3.0343
Subject(s) - seawater , assimilation (phonology) , diatom , vitamin , algae , chemistry , environmental chemistry , biology , food science , ecology , biochemistry , philosophy , linguistics
A new method for assaying vitamin B 12 in seawater using the marine diatom Cyclotella nana Hustedt is described. The criterion of response to variations in B 12 concentration was C 14 assimilation (added as Na 2 C 14 O 3 ) after 24‐hr exposure to the vitamin. C 14 assimilation was proportional to the B 12 content of the medium in an ecologically useful range (0.1–6 µµ g B 12 /ml) .