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DEHYDROGENASE ACTIVITY UNDER HYDROSTATIC PRESSURE BY ISOLATED MITOCHONDRIA OBTAINED FROM ALLOMYCES MACROGYNUS 1, 2
Author(s) -
Hill Eddie P.,
Morita Richard Y.
Publication year - 1964
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1964.9.2.0243
Subject(s) - citric acid cycle , isocitrate dehydrogenase , hydrostatic pressure , succinate dehydrogenase , mitochondrion , dehydrogenase , tricarboxylic acid , chemistry , energy metabolism , biochemistry , metabolism , biology , enzyme , physics , thermodynamics , endocrinology
Isolated mitochondria were tested for their capacity to dehydrogenate various intermediates of the tricarboxylic acid (TCA) cycle in an optical pressure cell. It was found that during the time of pressurization at room temperature (ca. 26.5C) each of the intermediates (alpha‐ketoglutarate, succinate, oxalosuccinate, and isocitrate) was affected differently by the application of pressure. No activity could be demonstrated at 1,000 atm when alphaketoglutarate or isocitrate was employed. In all cases, increased hydrostatic pressure brought about a decrease in mitochondrial dehydrogenase activity. From the data presented, it is postulated that one of the mechanisms that brings about the expiration of Allomyces macrogynus at pressures of 600 atm or more is the interruption of its energy‐yielding mechanism in terms of the operation of the TCA cycle.