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NITROGEN METABOLISM IN LAKES I. MEASUREMENT OF NITROGEN FIXATION WITH N 15 1
Author(s) -
Neess John C.,
Dugdale Richard C.,
Dugdale Vera A.,
Goering John J.
Publication year - 1962
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1962.7.2.0163
Subject(s) - nitrogen , isotopes of nitrogen , nitrogen fixation , tracer , isotope , incubation , environmental chemistry , chemistry , mass spectrometry , stable isotope ratio , nitrogen cycle , environmental science , radiochemistry , chromatography , physics , biochemistry , organic chemistry , quantum mechanics , nuclear physics
A method for measuring the rates of nitrogen fixation in samples of lake water using N 15 as a tracer is described, and a detailed description of glassware required is provided. The nitrogen present in the water is removed by flushing with Hc‐O mixture and then replaced by the labeled nitrogen. After incubation and treatment, the isotope ratio in the organic material is determined on a mass spectrometer. Analyses of lake water and (NH 4 ) SO 4 as isotope ratio blanks are described and a résumé of statistical data from these analyses is presented.