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THE SPREAD PLATE AS A METHOD FOR THE ENUMERATION OF MARINE BACTERIA 1, 2
Author(s) -
Buck John D.,
Cleverdon Robert C.
Publication year - 1960
Publication title -
limnology and oceanography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.7
H-Index - 197
eISSN - 1939-5590
pISSN - 0024-3590
DOI - 10.4319/lo.1960.5.1.0078
Subject(s) - enumeration , rod , agar , agar plate , biology , bacteria , mathematics , paleontology , combinatorics , medicine , alternative medicine , pathology
A comparison was made of agar plates spread with glass rods and poured agar plates for the enumeration of bacteria in the waters of Fisher’s Island Sound, salinity 30‰. Spread plates were shown to be markedly superior. Highest counts were obtained by spreading, using rods treated with Desicote (a silicone solution) and incubating plates at 25°C, rather than at 16°C, and in air rather than in air with CO 2 content increased.