Open AccessMetagenomic investigation of bacteria associated with dental lesions: a cross-sectional study
Author(s) -
Ali Kazemtabrizi,
Azam Haddadi,
Mahmoud Shavandi,
Naser Harzandi
Publication year - 2020
Publication title -
medicina oral, patología oral y cirugía bucal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 56
eISSN - 1698-6946
pISSN - 1698-4447
DOI - 10.4317/medoral.23326
Subject(s) - biofilm , metagenomics , root canal , 16s ribosomal rna , microbiology and biotechnology , dentin , dentistry , biology , streptococcus mutans , root caries , bacteria , lactobacillus , tooth surface , medicine , gene , genetics
Dental caries is considered as one of the most significant global health problem over the world. Dental caries initiates from bacterial shifts within the supragingival biofilm, then a polymicrobial biofilm is formed on the surface of tooth, and finally various bacterial species aggregate in a complex-organized manner. The exploiting variability in 16S rRNA gene sequence has been considered as a cost-efficient high-throughput characterization approach in human oral microbiome investigations. The aim of this study is to characterize bacterial species associated with superficial dental biofilm, underlying carious dentine and root caries lesion by16S rRNA gene-based metagenomic analysis.