Open AccessIn vitro antidiabetic, aphrodisiac and antimicrobial properties of para-propoxybenzoic acid isolated from Acacia auriculiformis A. Cunn Ex. Benth (Fabaceae) stem bark
Author(s) -
Ekarika Johnson,
Richard Alexis Ukpe,
Emmanuel E. Etim,
Uwemedimoh F. Umoh,
Olorunfemi A. Eseyin,
Anwanabasi E. Udoh,
Tina Mboho,
Paschal C. Anthony
Publication year - 2022
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v20i3.25
Subject(s) - aphrodisiac , antimicrobial , traditional medicine , ethyl acetate , acacia auriculiformis , chemistry , bark (sound) , ethanol , chromatography , botany , acacia , biology , biochemistry , organic chemistry , medicine , ecology , alternative medicine , pathology
Purpose: To investigate the in vitro antidiabetic, aphrodisiac and antimicrobial properties of para-propoxybenzoic acid isolated from Acacia auriculiformis stem bark.
Methods: Powdered dried stem bark of A. auriculiformis was extracted with 70 % ethanol in water and the dried extract obtained was suspended in water and partitioned with ethyl acetate and n-butanol to give their respective soluble fractions. The ethyl acetate fraction being more active, in terms of in-vitro aphrodisiac, antidiabetic and antimicrobial properties, was subjected to chromatographic separation to obtain C1 identified as parapropoxybenzoic acid, which was also evaluated for in-vitro properties using standard procedures.
Results: 4-Propoxybenzoic acid showed a weak aphrodisiac property which was not significantly different (p < 0.05) from that of control (normal saline) for all the sexual parameters tested. The compound exhibited antidiabetic activity by dose-dependently inhibiting the actions of α-amylase with half-maximal concentration (IC50) value of 50 ± 0.45 μg/mL. In this regard, it was comparable to that of the standard, acarbose (30 ± 0.18 μg/mL). In the antimicrobial study, a concentration of 200 μg/mL of the compound gave the highest zone of inhibition of 18 ± 0.23 mm against Vibo cholriae (NCTC 5438)
Conclusion: The results indicate that the isolated compound, 4-propoxybenzoic, exhibits good antidiabetic and anti-microbial but weak aphrodisiac properties.