Open AccessCytotoxic effect of Leurius quinquestratus (scorpion) venom in different human cancer cell lines in vitro
Author(s) -
Wesam M. Salama,
Sabry El-Naggar
Publication year - 2022
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v20i2.18
Subject(s) - hela , cytotoxicity , apoptosis , cell culture , mtt assay , in vitro , cytotoxic t cell , ic50 , chemistry , microbiology and biotechnology , cell cycle checkpoint , cell , cancer cell , cell cycle , pharmacology , biology , cancer , biochemistry , genetics
Purpose: In this study, the cytotoxicity of scorpion Leurius quinquestratus crude venom (LQCV) was evaluated in vitro in selected human cancer cell lines.
Methods: Breast (MCF-7), hepatocellular (HepG-2), colon (CaCo-2), cervix (HeLa) and alveolar (A-549) adenocarcinoma cell lines were tested. MTT assay and median inhibition concentration (IC50), apoptotic assay, caspase 3, P53, Bcl-2 proteins and cell cycle were determined.
Results: 24 hrs post-treatment, CaCo-2 represented the most sensitive cell line (IC50 of 4.12 μg/mL). Due to the exposure to 1/10 IC50 of LQCV, the percentage of the apoptotic cells, caspase 3, and P53 proteins were increased significantly (P<0.05) while Bcl-2 was decreased in comparison to untreated cells. Treatment with LQCV induced cell cycle arrest at G1 and G2/M phases.
Conclusion: LQCV displays potent cytotoxicity against selected human cell lines in vitro. Thus, the material could become a potent agent for the management of some cancers.