Open AccessMiR-379 inhibits proliferation and induces apoptosis in multiple myeloma by targeting Y-box binding protein 1
Author(s) -
Yunyun Wang,
Dexiang Ji,
Guoan Chen
Publication year - 2020
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v19i9.7
Subject(s) - multiple myeloma , apoptosis , flow cytometry , western blot , cell growth , microbiology and biotechnology , cancer research , reverse transcription polymerase chain reaction , viability assay , chemistry , biology , messenger rna , gene , immunology , biochemistry
Purpose: To determine the effect of miR-379 in multiple myeloma.Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate the expression of miR-379 in multiple myeloma cells. The effect of miR-379 on multiple myeloma progression was investigated by cell counting, bromodeoxyuridine staining, flow cytometry and Western blot analysis. A potential target for miR-379 was determined using a luciferase reporter assay.Results: MiR-379 expression was reduced in multiple myeloma cells, while over-expression of miR-379 increased both cell viability and proliferation of these cells (p < 0.05). Moreover, miR-379 blocked cell cycle multiple myeloma cells and promoted apoptosis by decreasing Bcl-2 expression, and increasing the expression of cleaved caspase-3 and Bax. MiR-379 bound to Y-box binding protein 1 (YBX1) and reduced YBX1 mRNA and protein expression in multiple myeloma cells (p < 0.05).Conclusion: A YBX1-mediated tumor-suppressive role for miR-379 in multiple myeloma cells has been identified, suggesting a potential strategy for the treatment of multiple myeloma.
Keywords: MiR-379, Y-box binding protein 1, Multiple myeloma, Proliferation, Apoptosis