Open AccessApoptotic effect of astaxanthin from white shrimp shells on lung cancer A549 cells
Author(s) -
Supita Tanasawet,
Wanida Sukketsiri,
Pennapa Chonpathompikunlert,
Wanwimol Klaypradit,
Morakot Sroyraya,
Pilaiwanwadee Hutamekalin
Publication year - 2020
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v19i9.6
Subject(s) - a549 cell , apoptosis , chemistry , astaxanthin , cell cycle , flow cytometry , viability assay , cancer cell , litopenaeus , microbiology and biotechnology , biology , cancer , biochemistry , shrimp , genetics , fishery , carotenoid
Purpose: To investigate the anti-cancer potential of astaxanthin from Litopenaeus vannamei encapsulated in liposomes (ASX) to treat lung cancer A549 cells.Methods: Lung adenocarcinoma A549 cells were cultured and treated with ASX, following which cell viability and nuclear staining were performed. Generation of ROS was identified by the DCFH-DA assay while tetramethylrhodamine ethyl ester was used to determine the mitochondrial membrane potential. Flow cytometry was applied to investigate caspase-3/7 activity and cell cycle distribution.Results: ASX inhibited growth of A549 in a concentration- and time- dependent manner. The IC50 values at 24, 48 and 72 h were 53.73, 22.85, 17.46 μg/mL, respectively (p < 0.05). After incubation with ASX, the morphological changes were observed in A549 cells following Hoechst 33342/PI fluorescent staining. ASX increased ROS generation and was associated with the collapse of mitochondrial membrane potential, which subsequently triggered the activation of caspase-3/7 activity leading to apoptosis (p < 0.05). In addition, A549 cells accumulated in the G0/G1 phase.Conclusion: The results suggest that ASX is a valuable nutraceutical agent to target A549 lung cancer cells via ROS-dependent pathway as well as blockage of cell cycle progression.
Keywords: Astaxanthin, Litopenaeus vannamei, Lung cancer, A549, Apoptosis