Open AccessIn vitro cytotoxic and antimicrobial effects of a novel peroxysesquiterpene glucoside from the rhizomes of Cyperus rotundus L (Cyperaceae)
Author(s) -
M Sabir,
Kawkab Y. Saour,
Shwan Rachid
Publication year - 2020
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v19i2.16
Subject(s) - cyperus rotundus , ethyl acetate , chemistry , hela , rhizome , chromatography , column chromatography , high performance liquid chromatography , antimicrobial , chloroform , traditional medicine , biology , organic chemistry , biochemistry , in vitro , botany , medicine
Purpose: To study the antimicrobial and cytotoxic potential of a novel 3,9-peroxsesquiterpene-15-Oglucoside from Cyperus rotundus rhizomes, against HeLa cell line and selected strains of microorganisms.
Methods: The rhizomes were macerated with methanol and fractionated with n-hexane, chloroform, ethyl acetate and butanol. High-performance liquid chromatography (HPLC) was performed together with chemical analysis of the fractions. The 3,9-peroxysesquiterpene-15-O-glucoside was purified through column chromatography of the ethyl acetate fraction, and its purity was determined via reversephase HPLC. Structural elucidation was done with Infrared (IR), proton-nuclear magnetic resonance (HNMR), carbon-13 nuclear magnetic resonance (13C NMR), and mass spectrometry (MS) spectroscopic analyses.
Results: The isolated compound exhibited bactericidal and fungicidal activities against S. aureus and C. albicans at concentration, respectively, in the range of 32 – 100 μg/mL, while MTT assay results showed the cytotoxicity of the compound against eukaryotic (HeLa) cell line (IC50, 88.32 μg/mL).
Conclusion: The isolated metabolite from the methanol extract of C. rotundus rhizome exhibits bactericidal, fungicidal, and cytotoxic potential. However, further studies are required to ascertain its suitability for use as a therapeutic agent.
Keywords: Terpenoids, Column chromatography, Spectroscopy, Antimicrobial, Cytotoxicity, Cyperus rotundus