Open AccessImmunosuppressive effect of voacamine from Voacanga africana Stapf based on SPRi experiment
Author(s) -
Hong Xiang Li,
Yan Qiu Wang,
Zhao Jin,
Li Zhu,
Wen Ying Huai,
Rongqiang Liu,
Tian E. Zhang,
Yun Deng
Publication year - 2021
Publication title -
tropical journal of pharmaceutical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.209
H-Index - 36
eISSN - 1596-5996
pISSN - 1596-9827
DOI - 10.4314/tjpr.v18i9.15
Subject(s) - cytotoxicity , concanavalin a , chemistry , cell growth , surface plasmon resonance , docking (animal) , dissociation constant , cell , in vitro , microbiology and biotechnology , stereochemistry , biochemistry , biology , receptor , medicine , materials science , nursing , nanoparticle , nanotechnology
Purpose: To investigate the affinity of a bis-indole alkaloid - voacamine from Voacanga Africana Stapf for IL-2Rα - and its immunosuppressive effect on concanavalin A-induced T cell proliferation and lipopolysaccharide -induced B cell proliferation in vitro.
Methods: Surface plasmon resonance imaging (SPRi) was used to screen the target protein of voacamine, while CCK-8 kit was used to evaluate cytotoxicity. Mitogen-induced proliferation assay was carried out to assess the inhibitory effect of voacamine on Con A-induced T cell proliferation and LPSinduced B cell proliferation. The binding characteristics of voacamine were investigated using a binding model with IL-2Rα constructed based on molecular docking simulation.
Results: Voacamine had a high-affinity for IL-2Rα with an equilibrium dissociation constant (KD) of 1.85×10-8 M. Cytotoxicity data showed that voacamine did not exhibit cytotoxicity at concentrations lower than 0.32 µM. However, it exerted significant immunosuppressive effect on B cells at a lower concentration, but had no influence on proliferation of T cells. Autodock results indicate that voacamine has a good interaction with the enzyme active site.
Conclusion: Voacamine and its analogues exert influence on the immune system.