Open AccessMorphoanatomy of striated myofibers in streptozotocin-induced diabetic wistar rats: assuaging effect of combined doses of Vernonia amygdalina and Azadirachta indica
Author(s) -
Oshiozokhai Eboetse Yama,
Olawale Victor Adeteye,
T.O. Kusemiju,
O.M. Avidime,
Timothy Danboyi,
A. A. Odetola,
Khadija A. Mohammed
Publication year - 2020
Language(s) - English
Resource type - Journals
ISSN - 2467-8252
DOI - 10.4314/rejhs.v8i2.5
Subject(s) - vernonia amygdalina , azadirachta , medicine , streptozotocin , diabetes mellitus , metformin , glutathione peroxidase , histology , antioxidant , traditional medicine , pharmacology , endocrinology , catalase , oxidative stress , biology , biochemistry
Objective: This study aimed to evaluate the effects of Azadirachta indica and Vernonia amygdalina extracts on the morphoanatomy of striated myofibrils in diabetic rat models.
Methodology: Thirty Wistar rats randomly assigned into 5 groups of 6 rats/group were used. Group A received distilled water only, B (herbal) received A. indica (500 mg/kg/day) and V. amygdalina (400 mg/kg/day) simultaneously, C were diabetic rats, D were diabetic rats treated with herbal extracts combined and E, diabetic rats treated with metformin. Diabetes was induced with streptozotocin (70 mg/kg). Muscles glutathione peroxidase (GPx) and blood glucose levels were determined. The rats were sacrificed at the end of 60days treatment. The quadriceps femoris muscle harvested for histology.
Results: Diabetic herbal treated rats became euglycemic by the end of 8 weeks. GPx activity was significantly (p<0.05) elevated compared to control. The histology of skeletal muscle fibers of the diabetic rats treated with herbal formulation and metformin showed minimal level of damage.Conclusions: The findings in this study showed that the herbal formulation could be used in treatment of diabetes and in ameliorating the associated muscular cytoarchitectural alterations.
Keywords: diabetic, extract, glutathione, peroxidase